Lipid Analysis
Get Rapid Insight You Can Rely On
Our targeted approach employs LC-MS and GC-MS platforms to offer high specificity, sensitivity, and superior reproducibility of the lipidome across a range of samples and systems. Unlock the details you need to identify and quantify your lipids of interest through our established analytical packages
Explore How Easy Lipidomics Can Be
We’ve developed analytical packages designed for cost-effective, comprehensive coverage and fast response to answer your lipid questions. See our most popular lipid coverage below. Our portfolio is constantly growing, so check often or contact us about specific targets that are important to your project
Lipid Analysis
Targeted Analysis
Lipid Analysis
Targeted Analysis
What you get with our Analytical Packages
1. Sample Preparation
Simply collect, preserve and ship it to us. We optimize our extraction and methods with every sample matrix to give you the best results
2. Rigorous Quality Control
As part of our routine QC process, we include internal standard, blank control and final review signoff by management team
3. Statistics & Lipid Bioinformatics Analysis
Basic and/or advance statistics along with lipid bioinformatics provided
4. Quantitative & Qualitative Analysis
Lipid identity and quantitation provided
5. Delivery in 6 Weeks*
Delivery time based on when lab receives samples. *Earlier turn-around-time of two weeks is available for additional surcharge
6. Electronic Report
Report includes methodology, analysis details, sample amounts injected, replicates, lipid identity and quantitation of each sample in tabular format. Basic and/or advance statistics maybe included depending on service package purchased
Analytes important to your project can be added to any standard assay, and new or customed assays can be developed to suit your requirements. Contact our team to discuss your needs
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Lipid Analysis Packages
Comprehensive Fatty Acid Assay
Recommended for analysis of food, marine, plants, animals, oils, nutraceuticals, lubricants, algae, cells and other biological samples to identify, quantify and characterize total fat content
Technique: Uses FAMEs analysis via GC-FID and GC-MS
Fatty Acid Coverage
- Saturated fatty acids (SFA)
- Monounsaturated fatty acid (MUFA) - C16:1, C18:1, C20:1, C24:1
- Polyunsaturated fatty acid (PUFA)
- Omega-3 – Docosahexaaenoic acid (DHA); Docosapentaenoic acid (DPA); Eicosapentaenoic acid (EPA); alpha-linolenic acid (ALA); Stearidonic acid (SDA);
- Omega-6 – Arachidonic acid (ARA); Linoleic acid (LA)
- Conjugated fatty acids - conjugated linolenic acid (CLN) and conjugated linoleic acid (CLA)
- Short Chain Fatty Acids (C2-C12)
- Free Fatty Acids (medium chain C6-12 & long chain C14-28)
- Trans fatty acid
- Fatty Alcohols
- Hydrocarbons
- Squalene
Proximal Lipid Assay
Gain information on bulk or total lipids and its subclasses in food, plants, animals, edible/essential oils, algae, lubricants, cells, capsules, pharmaceutical or nutraceuticals and other biological samples
Technique: combination of gravimetric, TLC-FID, GC-FID, GC-MS and LC-MS
Lipid Coverage
- Total lipids or fat content
- Total triglycerides
- Total phospholipids
- Total omega-3 fatty acids
- Total omega-6 fatty acids
- Total PUFA – polyunsaturated fatty acid
- Total MUFA – monounsaturated fatty acid
- Total SFA - saturated fatty acids
- Total Galactolipids
- Total Sterols
Complex Lipid Assay
Gain information on system metabolism, signaling, composition, assess specific organelle function or phenotypic response to environmental factors (ie. climate conditions, treatment exposure, toxins, agricultural input, biotic and abiotic stressors), etc.
This is an effective tool to evaluate membrane and storage lipids in various biological systems (human, plants, animals, microbes, biomaterials, disease model system, cells)
Technique: High resolution accurate mass tandem mass spectrometry (HRAM-MS/MS)
Lipid Coverage
- PC – Phosphatidylcholine
- PE – Phosphatidylethanolamine
- PS – Phosphatidylserine
- PI – Phosphatidylinositol
- PA – Phosphatidic acid
- PG – Phosphatidylglycerol
- SM – Sphingomyelin
- CL – Cardiolipin
- MGDG – Monogalactosyldiacylglycerol
- SQDG – Sulfoquinovosyl-diacylglycerol
- DGDG – Digalactosyldiacylglycerol
- CER – Ceramides
- acylated sphingolipids with fatty acids
- TG – Triglyceride
- DG – Diglyceride
- MG – Monoglyceride
- WE – Wax esters
Premium Complex Lipid Assay
This is an advance type of lipid analysis designed to evaluate discreet subclasses of lipids with special modifications or linkages. It will provide you with greater insight and understanding of lipid metabolism and function across a range of biological systems
Technique: high resolution accurate mass tandem mass spectrometry (HRAM-MS/MS)
Lipid Coverage
- Plasmalogens
- Ether lipids
- Monomethylated Phosphatidylethanolamine (PE)
- Dimethylated phosphatidylethanolamine (PE)
- Fatty acid esters of hydroxy fatty acid (FAHFA)
- Monoacetyldiacylglycerols (MADAG)
- Acylated Phosphatidylglycerol (PG)
- Ether phosphatidylcholine (PC) or phosphatidylethanolamine (PE)
- 1,2 or 1,3 DG isomers
- Short chain triglycerides (TG)
- Lyso plasmalogens PC or PE
- Stereoisomers of PLs
- Medium chain TG sterols: campesterol, sitosterols, beta-sitosterols, etc.
Active Microbial Community Composition
This assay is an excellent tool to for evaluating the subclasses of living microbes in the soil to understand:
- climate change
- effectiveness of agriculture management practices
- remediation practices, industrial activities or chemical contamination on soil health
- soil microbial community composition; its interactions or metabolic activity
- show structure or diversity of microbial habitat condition; physiological status or micro niche
Technique: GC-MS, GC-FID and LC-MS
Lipid Coverage
- Hydroxyl fatty acids
- Bacterial fatty acids
- Total phospholipid fatty acids (PLFAs)
- Phospholipids / diglyceride (DG) ratio
- Plasmalogens / PLFA ratio
- CL / PG ratio
- PG / PLFA ratio
- Trans / cis monoenoic PLFA
- Sterols
Lipid classes & species important to your project can be added to any standard assay, and new assays can be developed to suit your requirements. Contact our team to discuss your needs
Accelerate Your Discovery
TARGETED ANALYSIS packages
Choose from our analytical packages designed for cost-effective, comprehensive coverage and fast response to answer your lipid questions
ACYLCARNITINES
Acylcarnitines play a crucial function in animals, plants and certain microorganisms where they breakdown and process long chain fatty acids and transport it into the mitochondria for energy production.
Acylcarnitines also contribute to lipid synthesis that can stabilize or alter membrane composition, impact neurotransmitter levels, modulate gene and proteins and serve as a diagnostic biomarker for inherited metabolic disorders.
This analysis will evaluate how acylcarnitine levels are related to mitochondrial function in various biological systems.
CAROTENOID
Carotenoids are the red, yellow and orange lipid-soluble pigments naturally found in fruits, fungi, bacteria, algae and plants. In plants, they absorb light used for photosynthesis and provide photo-protection from reactive oxygen species (ROS).
In animals including humans, carotenoids are obtained through diet. Its antioxidant and anti-inflammatory properties has potential benefits of reducing risk of certain cancers, eye and cardiovascular diseases. Its ability to modulate gene expression and cell signaling pathways has made it a target for therapeutic development of various diseases.
This assay will identify and quantify the various carotenoid pigments in biological samples
TERPENES
Terpenes are aromatic compounds produced in plants, some insects and animals. It’s one of the largest bioactive lipid groups involved in photosynthesis, hormone synthesis, membrane permeability, etc. and offers therapeutic benefits such as anti-microbial, anti-inflammatory, anti-cancer, anti-depression properties that improve mental and brain health.
This analysis covers up to 200 terpenes from the 4 major classes: monoterpene, cispeterpene, diterpene, triterpene in food and plant samples.
Identify and characterize complex classes of terpenes (size, side chains, functional groups), its potency and quality in samples.
PHENOLIC ACIDS
Phenolic acids are found in plants-based foods like seeds, fruits, vegetables and whole grains. It contributes to the organolepic properties like color, astringency, aroma and bitterness.
Phenolic acids can alter cell signaling pathways, preserve foods and have many health benefits like antioxidant, antitumor, antimicrobial, anti-inflammatory, as well as reduction in hypertension, cardiovascular diseases.
This assay will evaluate benzoic and cinnamic acid derivatives in plant or food samples. Phenolic profiling can be used as a chemical fingerprint for food authentication to trace geographic and botanic origin of products since phenolic acids are highly sensitive to environmental input.
HETEROCYCLIC AMINES (HCA)
Heterocyclic amines (HCAs) are chemical compounds produced when meats are cooked at high temperature such as grilling, charring or frying. HCAs are mutagenic and carcinogenic meaning they can causes changes in DNA and increase the risk of cancer.
Intake of HCAs have been linked to several types of cancers including colorectal, breast, prostate, pancreas, lung, stomach and esophagus. The level of HCAs formed are influenced by method of cooking, temperature, cooking time as well as type of meat, degree of browning and its fat content.
This analysis will evaluate cooked meat quality and safety by characterizing HCAs and levels present in sample
VITAMINS
(fat & water soluble)
Fat-soluble vitamins are readily dissolved in fats and stored in the body. These consists of Vitamin A, D, E and K. Water soluble vitamins like Vitamin B complex and C travel freely through the body and excess unused amounts are excreted in urine so it needs to be replenished regularly through diet.
This analysis will evaluate fat and water soluble vitamins and its derivatives in food samples to provide information on,
- the structure and properties of these vitamins to better understand its nutritional value and biofunction
- aid in developing better delivery systems to enhance bioavailability and stability of vitamins
OXYLIPINS
Oxylipins are generated from oxygenated polyunsaturated fatty acids (PUFAs) such as omega-3 and omega-6 fatty acids. They function as signaling mediators and hormones that regulate cellular and physiological processes like metabolism, immune response, clearing of inflammation and cell death.
The imbalance of oxylipins in the body has been attributed to inflammation processes, cardiovascular diseases and cancers. Analyzing levels of oxylipin can provide insights on pathological processes, offer diagnostic and therapeutic strategies for various diseases.
This assay characterizes oxylipins in biological samples to understand diseases linked to oxylipin signaling, metabolites derived from PUFAs, develop therapeutic targets that modulating oxylipin production
ALDEHYDES
Aldehydes are class of organic compounds generated from the breakdown of lipids. They play an important role in energy metabolism, signal transduction and lipid and protein modification. Excessive amounts of aldehyde production are toxic to cells and tissues where it interacts with proteins, nucleic acids and other biomolecules causing cellular damage and dysfunction.
The imbalance of aldehyde levels has been associated with Alzheimer’s disease, Parkinson’s disease, cancer and cardiovascular disease.
This assay will analyze levels of aldehydes in biological samples which can provide insight into disease states, biomarkers, progression and offer diagnostic and therapeutic strategies to mitigate the toxic effect of excessive aldehyde production.
OXIDIZED LIPIDS
This assay profiles lipids that have undergone oxidative modification leading to reactive oxygen species (ROS) and oxidative stress.
Although ROS plays a key role in cell signaling, immune defense and energy metabolism in normal physiological processes, excessive amounts is harmful to cells and tissues leading to oxidative stress and damaging biomolecules such as proteins, lipids and DNA. This has been associated to aging, cancers, neurodegenerative diseases, cardiovascular disease and diabetes.
Analyzing levels of oxidized lipids can offer information into disease states and progression; identify biomarkers and therapeutic strategies to address diseases associated with oxidative stress; evaluate efficacy of of antioxidant therapies aimed at reducing oxidative stress.
Lipid classes & species important to your project can be added to any standard assay, and new assays can be developed to suit your requirements. Contact our team to discuss your needs
Accelerate Your Discovery
Custom Lipid Analysis
Define what you need and what’s important for your project. We’re up for the challenge to support you every step of the way. Contact our team to chat
Samples We Work With
Food
Biological
Environmental
We recommend running at least 3 replicates of each sample for analysis in order to produce statistically significant results. Samples should be frozen as soon as possible to minimize further metabolism or degradation of lipids and shipped on dry ice.
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Why our Clients
Choose Lipid Insight
Award Winning State of the Art Analytical Methods
Save time and eliminate the hassle with our proven and peer reviewed effective methods to ensure you get the best results
The Latest Powerful Platforms, Methods & Techniques
Get reliable results and actionable insight you can trust by leveraging our best-in-class methods and advance technology
Multidisciplinary Expertise with Diverse Industry Collaborations
Work with the most passionate people in the field to accelerate and transform R&D into powerful commercial opportunities
Our Happy Clients!
Hear what some of our clients and collaborators have to say about working with Lipid Insight.
We would like to thank you for the great meeting we had last week. It was important for us to get to know you and see the capabilities of your lab. We all agree that your expertise is valuable and we are satisfied with your proposal taking into consideration the challenge of this analysis.
R&D Manager
Food Industry
He (Dr. Thomas) is a scientist par excellence who has built a reputation as the “go to” person for solving lipid analytical issues
Principal Investigator
Memorial University
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Speak to a lipid expert! Get more information on how lipid analysis can advance your research. Please use the form below to provide some information or email us at info@lipidinsight.com We’re happy to chat and see how we can take your project to the next level!
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See Other Services We Offer
Lipid Imaging
Visualize multiple classes of lipids with DESI-MS lipid imaging using both targeted and untargeted approach to reveal lipid spatial distribution
Data Analysis
Apply statistics & lipid bioinformatics to establish meaningful links between the lipidome, lipid metabolism, mechanism & phenotype
Consulting
Elevate your impact! We support and empower researchers and innovators to confidently accelerate their discovery